Year 2017, Volume 2, Issue 1
| Year : | 2017 |
| Volume : | 2 |
| Issue : | 1 |
| Authors : | Miodrag DIMITRIJEVIĆ, Sofija PETROVIĆ, Borislav BANJAC, Goran BARAĆ, Aleksandra YURIEVNA DRAGOVIĆ, Alexander MIHAILOVICH KUDRYAVTSEV, Desimir KNEŽEVIĆ |
| Title : | GENETIC VARIABILITY OF THE CEREAL (POACEAE) GERMPLASM COLLECTION MONITORED BY PROTEIN AND MOLECULAR MARKERS |
| Abstract : | All the new challenges that food production has been experienced, requires adequate response not only in wide agricultural practice, but also in modern breeding programs. Broadening genetic variability is indispensable to meeting the border set by climatic changes, land erosion, human population growth, and sustainable agriculture. Gathering genetic variability, forming and examining genetic collection are integral part of the task. A germplasm collection of 220 entries, consisting of cereal (Poaceae) genotypes has been formed. Genetic variability of wheat (Triticum sp.), barley (Hordeum sp.), and goat grass (Aegilops sp.), samples from the germplasm collection was analysed using gliadin blocks as protein markers, as well as, Random Amplified Polymorphic DNA (RAPD) markers. Gliadin allelic variation was notable within Triticum sp. samples, revealing not only genetic divergence, but also the origin and the structure of populations. Genotype variation and structure of populations of Hordeum sp. was followed by hordein allelic variation. Sampled population expressed heterogeneity from two to five genotypes per population sample. Landraces, old and modern varieties were separated in collection using hordein allelic variation, as well. A small, pilot, investigation was conducted on Aegilops sp. polymorphism using RAPD primers. Number and percentage of polymorphic loci, effective number of alleles, expected heterozygosity and Shannon’s information index were used to estimate genetic variation. |
| For citation : | Dimitrijević, M., Petrović, S., Banjac, B., Barać, G., Yurievna Dragović, A., Mihailovich Kudryavtsev, A., Knežević, D. (2017).genetic variability of the cereal (Poaceae) germplasm collection monitored by protein and molecular markers. AGROFOR International Journal, Volume 2. Issue No. 1. pp. 75-82. DOI: 10.7251/AGRENG1701075D |
| Keywords : | biodiversity, wheat, barley, Aegilops sp., genetic marker |
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ISSN 2490-3434 (Printed)
ISSN 2490-3442 (Online)
ISSN 2490-3442 (Online)
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